Guillen will play right field and bat fourth, manager Trey Hillman said. He expects Guillen to get at least two at-bats.
"Unless things go real fast and he wants to get three," Hillman said.
Guillen also will play on Monday against the San Francisco Giants, but will be off on Tuesday when the Royals travel to Tucson for a game against the Arizona Diamondbacks.
Hillman let Guillen set his own pace to get ready to be in the lineup. In a simulated game on Saturday at Surprise, he ripped a solid single.
Second baseman Mark Grudzielanek, another veteran who was held back, also will play in both games. He played his first game on Friday and had two plate appearances.
Lefties on display: John Bale and Jorge De La Rosa, battling for a left-handed spot in the rotation, had mixed results in Saturday's 8-2 loss to the Milwaukee Brewers.
Bale started and gave up three runs on six hits, including Mike Cameron's first-inning home run.
"Johnny showed good stuff. He got hit hard in spots, but I thought he pitched better than his line today," Hillman said. "De La Rosa has some delivery issues. He gets in it and he gets out of it. It's just a matter of continuing to repeat his delivery to get it where he wants."
De La Rosa gave up two runs (one earned) on two hits in three innings. He issued two walks.
Non-roster right-hander Chin-hui Tsao made it through just two-thirds of an inning and gave up three runs, two on Joe Dillon's home run.
"Today's the flattest I've seen him. Everything was flat and coming back over the plate," Hillman said.
Billy in a bump: Billy Butler's inexperience at first base showed during a fielding gaffe in the second inning.
After center fielder Joey Gathright went deep to catch J.J. Hardy's drive, runner Bill Hall could have been doubled up at second base, but no one was covering the bag. Second baseman Alberto Callaspo and shortstop Jason Smith were out on the grass in "double-cut" formation and Butler should have covered second base, Hillman said.
Instead Butler broke belatedly and bumped into Bale, also headed toward the uncovered base.
"The first baseman's got to float, he's got to be ready to get there. It's one of those things. Billy doesn't have a lot of time logged there and he tried to get there but threw a roadblock on John and I'm glad John's OK," Hillman said.
Butler had no problems at the plate, however. He was 2-for-2 with a walk and has hits in six of his last seven at-bats for a .435 average.
Up next: There's no Daylight Savings Time in Arizona so Sunday's game against the Cubs will start at 3:05 p.m. CT. Brett Tomko is the Royals' starting pitcher to be followed by Julio Pimentel, Yasuhiko Yabuta, Leon Nunez and Ron Mahay. The Cubs' starter is Ryan Dempster with Kerry Wood, Neal Cotts, Carmen Pignatiello, Kevin Hart and Carlos Marmol also scheduled.
Dick Kaegel is a reporter for MLB.com. This story was not subject to the approval of Major League Baseball or its clubs.