DeJesus aggravated an injury to his right ankle, which he sprained in last Monday night's Cactus League game against the Los Angeles Dodgers. He missed four games but played seven innings on Saturday at Milwaukee.
"It happened on the ball that went over his head [Carlos Guillen's double] and he planted to go," manager Trey Hillman said.
DeJesus said he just "tweaked" the previous injury.
"It didn't feel great so I just tried to run it off," DeJesus said. "Nothing's broken or anything like that."
DeJesus led off against the Tigers and grounded out to Guillen at first. He made a catch in the first inning and chased a couple of hits in the second inning.
When DeJesus' turn to bat came in the third inning, Joey Gathright pinch-hit for him.
Dick Kaegel is a reporter for MLB.com. This story was not subject to the approval of Major League Baseball or its clubs.