The Royals outfielder, who has been a catalyst for the offense all season, was held out of Sunday's 5-4 win over the Twins because of a sore hip. DeJesus was, however, in uniform and on the bench.
"He's not moving very well," Royals manager Trey Hillman said. "That's why I took him out [Saturday] night."
DeJesus started Saturday's game and stole his 10th base in the first inning, which tied his career high. But he was lifted in the eighth inning after going 0-for-3 with a walk.
DeJesus had returned to the lineup Friday after missing the previous five games with a sore back.
"It's probably a result from the stiffness in the back," Hillman said.
With an off-day on Monday, the Royals would love to get DeJesus back in the lineup for the start of a nine-game road trip on Tuesday in Chicago.
"That's what I'm hoping for," Hillman said.
Robert Falkoff is a reporter for MLB.com. This story was not subject to the approval of Major League Baseball or its clubs.