Hosmer strained his right shoulder in the second inning, when he lunged for a sharply hit ball by Quintin Berry that went into the right-field corner for a triple. He came out of the game in the third and was replaced at first by Brayan Pena, a catcher by trade.
"I just kind of reached back and landed awkwardly on it. Hopefully, it's not serious. Nothing is torn," Hosmer said. "[I'll] just take it day by day. If it doesn't feel better, I'll get an MRI and check it out."
Escobar banged up his right shoulder diving for Jhonny Peralta's bloop hit in the seventh inning. Tony Abreu pinch-hit for Escobar in the eighth and then replaced him at shortstop.
"Hoz pinched his cuff in the back, Esky in the front. So we'll just go day to day with it," manager Ned Yost said.
Yost added that both would sit out Friday night's series opener at Cleveland, although Escobar thinks he could play.
"When I went down, I hit the front part of my shoulder," Escobar said. "I think I'll play tomorrow. I want to play."
Dick Kaegel is a reporter for MLB.com. This story was not subject to the approval of Major League Baseball or its clubs.